Part:BBa_K1679030:Design

J23119 + I13504 + J23119 + K115002 + E1010

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 933
  Illegal NheI site found at 956
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1582
  Illegal AgeI site found at 1694
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 705
  

Design Notes

The same promoter can control the quantity of transcription, so that the ratio of GFP and mCherry is related to RNA thermometer.

Source

Assembled parts from Registry through 3A assembly.